Journal: Mediators of Inflammation

Article Title: A Peptide Analogue of Selectin Ligands Attenuated Atherosclerosis by Inhibiting Monocyte Activation

doi: 10.1155/2019/8709583

Figure Lengend Snippet: IELLQAR decreases PLCA-induced atherosclerosis and impaired Ly-6C high monocyte recruitment to the arterial walls of ApoE −/− mice. Eight-week-old male ApoE-deficient mice randomized into four treatment groups, sham group, normal saline (NS), and two IELLQAR treatment groups (1 and 3 mg/kg), and fed a normal chow diet for 4 weeks. In PLCA surgery, three (left external carotid, internal carotid, and occipital artery) of the four branches of ApoE −/− mice were ligated, and only the superior thyroid artery was left to provide blood circulation. (a) Representative histological analysis results of the carotid artery following H&E staining ( n ≥ 5). Scale bar: 50 μ m. (b) Flow cytometric dot plots showing the percentage of CD45 + CD11b + Ly-6C high monocytes in circulation. (c) Representative histological analysis results of the carotid artery following immunofluorescence staining with Ly-6C ( n ≥ 5). Scale bar: 50 μ m and 25 μ m, respectively. (d) The mRNA expression levels of MCP-1, CCR2, and ICAM-1 in carotid artery tissue were measured by qRT-PCR ( n ≥ 5). Data are presented as the mean ± SEM; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Primer sequences for quantitative real-time polymerase chain reaction (qRT-PCR) analysis were designed and purchased from GenePharma Co. Ltd. (Shanghai, China).

Techniques: Staining, Immunofluorescence, Expressing, Quantitative RT-PCR

Journal: Mediators of Inflammation

Article Title: A Peptide Analogue of Selectin Ligands Attenuated Atherosclerosis by Inhibiting Monocyte Activation

doi: 10.1155/2019/8709583

Figure Lengend Snippet: IELLQAR inhibited monocyte activation by P-selectin-dependent activation of the NF- κ B pathways. (a) PBMCs were treated with P-selectin (10 μ g/mL) for different times. Representative Western blots showing the expression levels of total and phosphorylated I κ B α and P65. (b) PBMCs were treated with IELLQAR (1, 3, or 10 μ M) and coincubated with or without 10 μ g/mL of P-selectin for 30 min. Representative Western blots showing the expression levels of total and phosphorylated I κ B α and P65. (c) PBMCs were treated with PBS, LPS (1 μ g/ml), P-selectin, IELLQAR (10 μ M) plus P-selectin, or IELLQAR plus LPS for 2 h, and the mRNA expression levels of IL-6, TNF- α , and MCP-1 were measured by qRT-PCR. Data are presented as the mean ± SEM ( n = 3). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Primer sequences for quantitative real-time polymerase chain reaction (qRT-PCR) analysis were designed and purchased from GenePharma Co. Ltd. (Shanghai, China).

Techniques: Activation Assay, Western Blot, Expressing, Quantitative RT-PCR